Composite

Part:BBa_K3440002:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


mCherry under prma promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to test the leakiness of prma promoter. A reporter mCherry gene was added to monitor expression. A moderate strength RBS (BBa_B0034) was used.

Source

mCherry from Anaplasma marginale

References